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Rikcha:Summary of SAGE.svg

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T'iktuna
English: Within the organisms, genes are transcribed and spliced (in eukaryotes) to produce mature mRNA transcripts (red). The mRNA is extracted from the organism and reverse transcriptase is used to copy the mRNA into stable ds-cDNA (blue). In SAGE, the ds-cDNA is digested by restriction enzymes (at location ‘X’ and ‘X’+11) to produce 11-nucleotide ‘digitag’ fragments. These digitags are concatenated and sequences using long-read sanger sequencing (different shades of blue indicate digitags from different genes). The sequences are deconvoluted to find the occurrence number of each digitag. Digitag can be used to report on transcription of the gene that the digitag came from is known.[1]
P'unchaw
Pukyu Kikinpa rurasqan
Ruraq Thomas Shafee
Huk musuqchasqakuna

Also in series:

  • RNA Microarray
    RNA Microarray
  • RNA-Seq
    RNA-Seq
  • SAGE
    SAGE

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w:es:Creative Commons
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Este archivo está disponible bajo la licencia Creative Commons Atribución 4.0 Internacional.
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Kay phatakuna tukuptinqa:
  • Ruraqpa sutinta willay – Kay rurasqataqa ruraqninpa icha saqillaqninpa sut'ichasqan hina unanchanaykim (ichataq amapuni kay hinachu, pay q'imisunkiman icha rurayniykita q'iminman rikch'akunman).
  1. ↑ Lowe, Rohan (2017-05-18). "Transcriptomics technologies". PLOS Computational Biology 13 (5): e1005457. DOI:10.1371/journal.pcbi.1005457. PMID 28545146. PMC: PMC5436640. ISSN 1553-7358.

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representa a Español Simi

Serial analysis of gene expression Ingles Simi

creador Español Simi

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nombre del autor Español Simi: Thomas Shafee
URL Español Simi: https://commons.wikimedia.org/wiki/user:Evolution_and_evolvability
nombre de usuario en Wikimedia Español Simi: Evolution and evolvability

fecha de fundación o creación Español Simi

5 ukt 2016

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kunan12:21 13 phi 201712:21 13 phi 2017-pa musuqchasqanmanta uchuylla rikchacha938 × 756 (180 kB)Evolution and evolvabilitytext as paths to avoid artefacts
10:48 13 phi 201710:48 13 phi 2017-pa musuqchasqanmanta uchuylla rikchacha938 × 756 (104 kB)Evolution and evolvabilityupdate sequence text to 'sans-serif' open font for more robust rendering
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